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Statistiche

Oggi28
Ieri252
Questa settimana280
Questo mese5327
Totale956424

Martedì, 22 Agosto 2017

Visitatori: 33 visitatori online.
ABSTRACT Shotgun metagenomics provides a powerful assumption-free approach to the recovery of pathogen genomes from
contemporary and historical material. We sequenced the metagenome of a calcified nodule from the skeleton of a 14th-century
middle-aged male excavated from the medieval Sardinian settlement of Geridu. We obtained 6.5-fold coverage of a Brucella
melitensis genome. Sequence reads from this genome showed signatures typical of ancient or aged DNA. Despite the relatively
low coverage, we were able to use information from single-nucleotide polymorphisms to place the medieval pathogen genome
within a clade of B. melitensis strains that included the well-studied Ether strain and two other recent Italian isolates. We con-
firmed this placement using information from deletions and IS711 insertions. We conclude that metagenomics stands ready to
document past and present infections, shedding light on the emergence, evolution, and spread of microbial pathogens.ABSTRACT Shotgun metagenomics provides a powerful assumption-free approach to the recovery of pathogen genomes from
contemporary and historical material. We sequenced the metagenome of a calcified nodule from the skeleton of a 14th-century
middle-aged male excavated from the medieval Sardinian settlement of Geridu. We obtained 6.5-fold coverage of a Brucella
melitensis genome. Sequence reads from this genome showed signatures typical of ancient or aged DNA. Despite the relatively
low coverage, we were able to use information from single-nucleotide polymorphisms to place the medieval pathogen genome
within a clade of B. melitensis strains that included the well-studied Ether strain and two other recent Italian isolates. We con-
firmed this placement using information from deletions and IS711 insertions. We conclude that metagenomics stands ready to
document past and present infections, shedding light on the emergence, evolution, and spread of microbial pathogens.ABSTRACT Shotgun metagenomics provides a powerful assumption-free approach to the recovery of pathogen genomes from
contemporary and historical material. We sequenced the metagenome of a calcified nodule from the skeleton of a 14th-century
middle-aged male excavated from the medieval Sardinian settlement of Geridu. We obtained 6.5-fold coverage of a Brucella
melitensis genome. Sequence reads from this genome showed signatures typical of ancient or aged DNA. Despite the relatively
low coverage, we were able to use information from single-nucleotide polymorphisms to place the medieval pathogen genome
within a clade of B. melitensis strains that included the well-studied Ether strain and two other recent Italian isolates. We con-
firmed this placement using information from deletions and IS711 insertions. We conclude that metagenomics stands ready to
document past and present infections, shedding light on the emergence, evolution, and spread of microbial pathogens.ABSTRACT Shotgun metagenomics provides a powerful assumption-free approach to the recovery of pathogen genomes from
contemporary and historical material. We sequenced the metagenome of a calcified nodule from the skeleton of a 14th-century
middle-aged male excavated from the medieval Sardinian settlement of Geridu. We obtained 6.5-fold coverage of a Brucella
melitensis genome. Sequence reads from this genome showed signatures typical of ancient or aged DNA. Despite the relatively
low coverage, we were able to use information from single-nucleotide polymorphisms to place the medieval pathogen genome
within a clade of B. melitensis strains that included the well-studied Ether strain and two other recent Italian isolates. We con-
firmed this placement using information from deletions and IS711 insertions. We conclude that metagenomics stands ready to
document past and present infections, shedding light on the emergence, evolution, and spread of microbial pathogens.
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